DOI:10.6661/TESFE.2011012台灣昆蟲Formosan Entomol. 31: 149-156 (2011) 綜合論述  Review Article
Formosan Entomologist
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Behind the Scenes of an Ant Genome Project 【Review Article】

Yannick Wurm*
Yannick Wurm*
*通訊作者E-mail :
Received: 2011/04/28     Accepted: 2011/04/30     Available online: 2010/06/01
Dramatic improvements in DNA sequencing technologies have led to a more than 1,000-fold reduction in sequencing costs over the past five years. Genome-wide research approaches can thus now be applied beyond medically relevant questions to examine the molecular-genetic basis of behavior, development and unique life histories in almost any organism. A first step for an emerging model organism is usually establishing a reference genome sequence. I offer insight gained from the fire ant genome project. First, I detail how the project came to be and how sequencing, assembly and annotation strategies were chosen. Subsequently, I describe some of the issues linked to working with data from recently sequenced genomes. Finally, I discuss an approach undertaken in a follow-up project based on the fire ant genome sequence.


Key words: genome assembly, how-to, genome annotation, fire ant, Solenopsis invicta
關鍵詞: 基因體組裝、指引、基因體註解、火蟻、入侵紅火蟻。
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