DOI:10.6661/TESFE.2011012台灣昆蟲Formosan Entomol. 31: 149-156 (2011) 綜合論述  Review article
Formosan Entomologist
Journal Homepage: entsocjournal.yabee.com.tw
Behind the Scenes of an Ant Genome Project 【Review article】

火蟻基因組計畫的幕後側寫【綜合論述】
Yannick Wurm*
Yannick Wurm*
*通訊作者E-mail : yannick.wurm@unil.ch
Received: 2011/04/28     Accepted: 2011/04/30     Available online: 2010/06/01
Abstract
Dramatic improvements in DNA sequencing technologies have led to a more than 1,000-fold reduction in sequencing costs over the past five years. Genome-wide research approaches can thus now be applied beyond medically relevant questions to examine the molecular-genetic basis of behavior, development and unique life histories in almost any organism. A first step for an emerging model organism is usually establishing a reference genome sequence. I offer insight gained from the fire ant genome project. First, I detail how the project came to be and how sequencing, assembly and annotation strategies were chosen. Subsequently, I describe some of the issues linked to working with data from recently sequenced genomes. Finally, I discuss an approach undertaken in a follow-up project based on the fire ant genome sequence.

摘要
近五年DNA定序技術的進展已使得定序成本比過去減少1,000倍以上。因此全基因體研究的方法不僅應用於解決醫學相關問題,也能進一步探討大部分生物的基礎生物學,包括以分子遺傳為主之行為模式、發育及獨特之活史。以新興模式生物為例,首要步驟即為建立參考基因組序列。本文將提供以火蟻基因體定序計畫所得之啟發。首先,詳述此計畫的緣由,並介紹如何選擇基因體解序、組裝和基因體註解的策略。接著說明如何將火蟻基因體資料與近來被解序之物種作結合。最後,討論以火蟻基因體序列為基礎,進行後續研究計畫的方法。

Key words: genome assembly, how-to, genome annotation, fire ant, Solenopsis invicta
關鍵詞: 基因體組裝、指引、基因體註解、火蟻、入侵紅火蟻。

下載其它卷期全文 Browse all articles in archive: http://entsocjournal.yabee.com.tw